Description of Research Expertise

Research Interests

Discovery and characterization of diagnostic and therapeutic monoclonal antibodies

Research Summary

My laboratory studies human immune repertoires in health and disease using phage display technologies for the production of human and non-human monoclonal antibodies relevant to transfusion medicine, benign hematology, infectious diseases, and oncology. Current studies focus on developing targeted drug delivery methods using red cell antibodies coupled to novel anti-thrombotic/anti-inflammatory molecules; cloning monoclonal primate neutralizing Dengue, Ebola, MERS and Zika virus antibodies for human in vivo antibody gene expression therapy; and isolating recombinant human and humanized antibody fragments for the design of T-cell chimeric antigen receptors for cancer therapy.

Selected Publications

Bhagwat, A.S., Torres, L., Shestova, O., Shestov, M., Mellors, P.W., Fisher, H.R., Farooki, S.N., Frost, B.F., Loken, M.R., Gaymon, A.L., , Frazee, D., Rogal, W., Frey, N., Hexner, E.O., Luger, S.M., Loren, A.W., Martin, M.E., McCurdy, S.R., Perl, A.E., Stadtmauer, E., Brogdon, J.L., Fraietta, J.A., Hwang, W.T., Siegel, D.L., Plesa, G., Aplenc, R., Porter, D.L., June, C.H., Gill, S.I.: Cytokine-mediated CAR T therapy resistance in AML Nature Medicine in press : 2024.

Mack, E.A., Dougher, M.C., Ginda, A., Cahill, C., Murter, M., Schell, K., Tanhehco, Y.C., Siegel, D.L., Bhoj, V., Fesnak, A.D., Kambayashi, T., Aqui, N., O’Doherty, U.: Red cell exchange for rapid leukoreduction in adults with hyperleukocytosis and leukostasis Blood 143 : 1049-1054,2024.

Bagley, S.J., Logun, M., Fraietta, J.A., Wang, X., Desai, A.S., Bagley, L.J., Nabavizadeh, A., Jarocha, D., Martins, R., Maloney, E., Lledo, L., Stein, C., Marshall, A., Leskowitz, R., Jadlowsky, J.K., Christensen, S., Oner, B.S., Plesa, G., Brennan, A., Gonzalez, V., Chen, F., Sun, Y., Gladney, W., Barrett, D., Nasrallah, M.P., Hwang, W.-T., Ming, G.-L., Song, H., Siegel, D.L., June, C.H., Hexner, E.O., Binder, Z.A., O'Rourke, D.M.: Intrathecal bivalent CAR T cells targeting EGFR and IL13Rα2 in recurrent glioblastoma: Phase 1 trial interim results Nature Medicine 30 : 1320-1329,2024.

Jung, I.-Y., Noguera-Ortega, E., Bartoszek, R., Collins, S.M., Williams, E., Davis, M., Jadlowsky, J.K., Plesa, G., Siegel, D.L., Chew, A., Levine, B.L., Berger, S.L., Moon, E.K., Albelda, S.M., Fraietta, J.A.: Tissue-resident memory CAR T cells with stem-like characteristics display enhanced efficacy against solid and liquid tumors Cell Reports Medicine 4 : 101053,2023.

Halkidis, K., Meng, C., Liu, S., Mayne, L., Siegel, D.L., Zheng, X.L.: Mechanisms of inhibition of human monoclonal antibodies in immune thrombotic thrombocytopenic purpura Blood 141 : 2993-3005,2023.

Yoshimoto, S., Chester, N., Xiong, A., Radaelli, E., Wang, H., Brillantes, M., Gulendran, G., Glassman, P., Siegel, D.L., Mason, N.J: Development and pharmacokinetic assessment of a fully canine anti-PD-1 monoclonal antibody for comparative translational research in dogs with spontaneous tumors mAbs 15 : e2004638-1 to e2004638-11,2023.

Garfall, A., Cohen, A., Susanibar-Adaniya, S., Hwang, W.-T., Vogl, D., Waxman, A., Lacey, S., Gonzalez, V., Fraietta, J.A., Gupta, M., Kulikovskaya, I., Tian, L., Chen, F., Koterba, N., Bartoszek, R.L., Patchin, M., Xu, R., Plesa, G., Siegel, D.L., Brennan, A., Nelson, A.-M., Ferthio, R., Cosey, A., Shea, K.-M., Leskowitz, R., Four, M., Wilson, W., Miao, F., Lancaster, E., Carreno, B., Linette, G., Hexner, E., Young, R., Bu, D., Mansfield, K., Brogdon, J., June, C., Milone, M.C., Stadtmauer, E.: Anti-BCMA/CD19 CAR T Cells with early immunomodulatory maintenance for multiple myeloma responding to initial or later-line yherapy Blood Cancer Discovery 4 : 118-133,2023.

Jung, I.-Y., Bartoszek, R.L., Rech, A., Collins, S.M., Ooi, S.-K., Williams, E.F., Hopkins, C., Narayan, V., Haas, N.B., Frey, N.V., Hexner, E.O., Siegel, D.L., Plesa, G., Porter, D.L., Cantu, A., Everett, J.K., Guedan, S., Berger, S.L., Bushman, F.D., Herbst, F., Fraietta, J.A.: Type I interferon signaling via the EGR2 transcriptional regulator potentiates CAR T cell-intrinsic dysfunction Cancer Discovery 13 : 1636-1655,2023.

Feng, Z., He, X., Zhang, X., Wu, Y., Xing, B., Knowles, A., Shan, Q., Miller, S., Hojnacki, T., Ma, J., Katona, B.W., Gade, T.P.F., Siegel, D.L., Schrader, J., Metz, D.C., June, C.H., Hua X.: Potent suppression of neuroendocrine tumors and gastrointestinal cancers by CDH17CAR T cells without toxicity to normal tissues Nature Cancer 3 : 581-594,2022.

Narayan, V., Barber-Rotenberg, J.S., Jung, I.Y., Lacey, S.F., Rech, A.J., Davis, M.M., Hwang, W.-T., Lai, P., Carpenter, E.L., Maude, S.L., Plesa, G., Vapiwala, N., Chew, A., Moniak, M., Sebro, R.A., Farwell, M.D., Marshall, A., Gilmore, J., Lledo, L., Dengel, K., Church, S.e., Hether, T.D., Xu, J., Gohil, M., Buckingham, T.H., Yee, S.S., Gonzalez, V.E., Kulikovskaya, I., Chen, F., Tian, L., Tien, K., Gladney, W., Nobles, C.L., Raymond, H., Prostate Cancer Cellular Therapy Program Investigators, Hexner, E.O., Siegel, D.L., Bushman, F.D., June, C.H., Fraietta, J.A., Haas, N.B.: PSMA-redirected TGFbeta-insensitive armored CAR T cell to treat metastatic castration resistant prostate cancer Nature Medicine 28 : 724-734,2022.

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Academic Contact Information

9-112 South Tower, Big 421
3400 Civic Center Blvd
Philadelphia, PA 19104
Phone: 215-662-3942