Penn Medicine Provider
Pathology and Laboratory Medicine
Eline T. Luning Prak, MD, PhD
Sees patients age 18 and up
Penn Pathology and Laboratory Medicine HUP
Call 800-789-7366

About me

  • Professor of Pathology and Laboratory Medicine at the Hospital of the University of Pennsylvania

Molecular Immunology- I have expertise in next-generation sequencing-based assays of immune repertoires and computational analysis of the immune repertoire.  I direct a research lab that performs immune repertoire profiling of antibody gene rearrangements in B cells and T cell receptor gene rearrangements in T cells.  Immune repertoire profiling can be used clinically for the evaluation of malignant and non-malignant clonal expansions, clone tracking, minimal residual disease assessment, evaluation of immune responses and immunologic disorders ranging from immunodeficiency to autoimmunity.  I am the past-president of the Adaptive Immune Receptor Repertoire (AIRR) Community, an international group of investigators who study immune repertoires and share data. 

Flow Cytometry- I have expertise in clinical and translational immunophenotyping experiments.  I direct the Human Immunology Core which develops and performs multicolor immunophenotyping panels and other translational immunology assays for early-phase clinical trials.  I am familiar with human B cell lymphocyte maturation, but also provide input into the design of immunophenotyping panels for malignant B and T cell disorders.  I also serve as a consultant to HUP allergy immunology physicians who use clinical flow cytometry data as part of an immunodeficiency evaluation.

Autoimmune and infectious disease serology- I have expertise in autoimmune serology, autoantibody profiling and serologic assays for infectious diseases including SARS-CoV-2 and lyme disease.  I am the Associate Director of the Clinical Immunology laboratory at the Hospital of the University of Pennsylvania, which performs a wide range of clinical assays.

HLA typing and allosensitization evaluations-  I have expertise in HLA and anti-HLA immune responses, and provide input into clinical test design, evaluation and test reporting for the HLA lab at the Hospital of the University of Pennsylvania.

Education and training

  • Medical School: University of Pennsylvania School of Medicine
  • Residency: Hospital of the University of Pennsylvania
  • Fellowship: Hospital of the University of Pennsylvania

Insurance accepted

My Locations

Learn about location

Penn Medicine hospital privileges

  • Hospital of the University of Pennsylvania: Has privileges to treat patients in the hospital.
  • Pennsylvania Hospital: Has privileges to treat patients in the hospital.
  • Penn Presbyterian Medical Center: Has privileges to treat patients in the hospital.
Dr. Luning Prak is a Penn Medicine physician.

Qualifications and experience

My research

Golden, G. J., Wu, V.H., Hamilton, J.T., Amses, K., Japp, A.S., Liu, C., Pampena, M.B., Kuri-Cervantes, L., Knox, J.J., Gardner, J.S., HPAP Consortium, Atkinson, M.A., Brusko, T.M., Luning Prak, E.T., Kaserner, K.H., Naji, A., and M.R. Betts Immune perturbations in human pancreas lymphatic tissues prior to and after type 1 diabetes onset , Nature Communications: 2025

Knox, J.J., Lee, I., Rosenfeld, A.M., Meng, W., Liu, F., Kearns, C., O'Doherty, U., Shaked, A., Olthoff, K.M. and E.T. Luning Prak B cell subset representation predicts COVID-19 vaccine response in solid organ transplant recipients , The Journal of Infectious Diseases: 2025

S. J. Hanna, R. H. Bonami, B. Corrie, M. Westley, A. L. Postal, E.T. Luning Prak, F. Breden, A.W. Michels, T.M. Brusko, and on behalf of the Type 1 Diabetes AIRR Consortium The Type 1 Diabetes T cell Receptor and B cell Receptor Repository in the AIRR Data Commons: A Practical Guide for Access, Use, and Contributions Through the Type 1 Diabetes AIRR Consortium , Diabetologia, 68(1): 2024,186-202

Fu, J., Hsiao, T., Waffarn, E., Meng, W., Long, K.D., Frangaj, K., Jones, R., Gorur, A., Shtewe, A., Li, M., Muntnich, C.B., Rogers, K., Jiao, W., Velasco, M., Matusmoto, R., Kubota, M., Wells, S., Danzl, N., Ravelo, S., Luga, A., Vasilescu, E.R., Griesemer, A., Weiner, J., Farber, D.L., Luning Prak, E.T., Martinez, M., Kato, T., Hershberg, U. and M. Sykes Dynamic establishment and maintenance of the human intestinal B cell population and repertoire following transplantation in a pediatric-dominated cohort , Frontiers in Immunology, 15: 2024

Anderson, E., Powell, M., Yang, E., Kar, A., Leung, T., Sison, C., Steinberg, R., Mims, R., Choudhury, A., Espinosa, C., Okoye, N.C., Choi, E.J., Marder, G., Narain, S., Gregersen, P.K., Mackay, M., Diamond, B., Levy, T., Zanos, T.P., Khosroshanhi, A., Sanz, I., Luning Prak, E.T., Bar-Or, A., Merrill, J., Arriens, C., Pardo, G., Guthridge, J., James, J., Payne, A., Utz, P.J., Boss, J.M., Aranow, C. and A. Davidson Factors associated with immune responses to SARS-CoV-2 vaccination in individuals with autoimmune diseases , JCI Insight, 9(13): 2024

Chong, E.A., Kumashie, K.G., Chong, E.R., Fabrizio, J., Gupta, A., Svoboda, J., Barta, S.K., Walsh, K.M., Napier, E.B., Lundberg, R.K., Nasta, S.D., Gerson, J.N., Landsburg, D.J., Gonzalez, J., Gaano, A., Weirock, M.E., McAllister, C.M., Awofalaju, M., John, G.N., Kammerman, S.C., Novaceck, J., Pajarillo, R., Lundgreen, K.A., Tanenbaum, N., Douma, S., Drapeau, E.M., Adamski, S., D'Andrea, K., Pattekar, A., Hicks, A., Korte, S., Sharma, H., Herring, S., Williams, J.C., Hamilton, J.T., Bates, P., Hensley, S.E., Luning Prak, E.T., Greenplate, A.R., Wherry, E.J., Schuster, S.J., Ruella, M. and L.A. Vella Immunologic predictors of vaccine responsiveness in patients with lymphoma and CLL , Journal of Infectious Diseases: 2024

T. Narasaraju, I. Neeli, S.L. Criswell, A. Krishnappa, W. Meng, V. Silva, G. Bila, V. Volk, Z. Serhiy, G.L. Bowlin, N. Meyer, E. T. Luning Prak, M. Radic, R. Bilyy. Neutrophil activity and extracellular matrix degradation: drivers of lung tissue destruction in fatal COVID-19 cases and implications for long COVID , Biomolecules, 14(236): 2024

Horvath, S., Huang, Z., Koelper, N.C., Martinez, C., Tsao, P.Y., Zhao, L., Goldberg, A.B., Hannum, C., Putt, M.E., Luning Prak*, E.T. and C.A. Schreiber*, (*equal contributions, co-senior authors) Induced abortion and the risk of Rh sensitization , JAMA, 330(12): 2023,1167-1174

Matsumoto, R., Gray, J., Rybkina, K., Oppenheimer, H., Levy, L., Friedman, L.M., Khamaisi, M., Meng, W., Rosenfeld, A.M., Guyer, R.S., Bradley, M., Chen, D., Atkinson, M.A., Brusko, T.M., Brusko, M., Connors, T.J., Luning Prak, E.T., Hershberg, U., Sims, P.A., Hertz, T. and D. L. Farber Induction of bronchus-associated lymphoid tissue is an early life adaptation for promoting human B cell immunity , Nature Immunology, 24(8): 2023,1370-1381

Rybkina, K., Bell, J.N., Bradley, M.C., Wohlbold, T., Scafuro, M., Meng, W., Korenberg, R.C., Davis-Porada, J., Anderson, B.R., Weller, R.J., Milner, J., Moscona, A., Poroto, M., Luning Prak, E.T., Pethe, K., Connors, T.J. and D.L. Farber SARS-CoV-2 infection and recovery in children: Distinct T cell responses in MIS-C compared to COVID-19 , Journal of Experimental Medicine, 220(8): 2023

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