Mathew Blurton-Jones, PhD; F. Chris Bennett, MD; Sonia Lombroso; and Jean Paul Chadarevian
PHILADELPHIA—A promising new approach may safely replace microglia—the only members of the immune system in the brain—according to new research conducted in mouse models by neuroscientists at the Perelman School of Medicine at the University of Pennsylvania and the University of California, Irvine. The researchers used a selective microglia-killing medicine to get rid of old microglia, while also replenishing them with transplanted surrogate cells in their place. These findings, published in the Journal of Experimental Medicine, may hold the potential for treating and even preventing neurodegenerative disorders, such as Alzheimer’s disease.
When microglia are healthy, they serve as the central nervous system’s resident front-line disease warriors. But there is evidence they can become dysfunctional in many neurological conditions.
“Until recently, scientists have mainly been looking at the mechanisms that drive microglial dysfunction and trying to find drugs to change their activity. But with this study, we’ve found a way to potentially harness microglia themselves to treat those diseases,” said Mathew Blurton-Jones, PhD, a professor of Neurobiology and Behavior at UCI.
“There is an obstacle because once our own microglia develop in the location where they are supposed to be in our brains, they don’t give up that space,” said F. Chris Bennett, MD, an assistant professor of Psychiatry at Penn. “They block the ability to deliver new cells that would take their place. If you want to insert donor microglia, you have to deplete the host microglia to open up room.”
Microglia depend on signaling by a protein on their surface called CSF1R for their survival. The FDA-approved cancer drug pexidartinib has been found to block that signaling, ablating them. This process would seem to offer a way to clear space in the brain to insert healthy donor microglia. However, there is a dilemma: unless the pexidartinib is stopped before the donor microglia are added, it will eliminate them, too. But once the drug is terminated, the host microglia regenerate too fast to effectively put in the donor cells.
“Our team believed that if we could overcome the brain’s resistance to accepting new microglia, we could successfully transplant them into patients using a safer, more effective process in order to target a great number of diseases,” said co-first author Sonia Lombroso, a Penn PhD student and member of the Bennett Lab. “We decided to investigate whether we could make the donor microglia resistant to the drug that eliminates their host counterparts.”
The researchers used CRISPR gene-editing technology to create one amino acid mutation, known as G795A, which they introduced into donor microglia produced from human stem cells or a mouse microglial cell line. Then they injected the donor microglia into humanized rodent models while administering pexidartinib, with exciting results.
“We discovered that this one small mutation caused the donor microglia to resist the drug and thrive, while the host microglia continued to die off,” said co-first author Jean Paul Chadarevian, a UCI PhD student who is a member of the Blurton-Jones Lab. “This finding could lead to many options for developing new microglial-based treatments. Pexidartinib is already approved for clinical use and appears to be relatively well tolerated by patients.”
Approaches could range from fighting disease by replacing dysfunctional microglia with healthy ones to designing microglia that can recognize imminent threats and strike against them with therapeutic proteins before they cause harm.
The Penn-UCI team believes treatments based on this kind of microglial method could be developed within a decade. Their next investigations include studying in rodent models how to use the approach to attack the brain plaques associated with Alzheimer’s and other similar diseases.
Support for the project was provided by the National Institutes of Health, National Science Foundation, The Paul Allen Frontiers Group, Klingenstein-Simons Fellowship Award in Neuroscience and the Susan Scott Foundation.
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