SAIF Optical Bioluminescence Services

The facility currently contains nine optical imaging systems in three locations. The instrumentation allows sensitive, noninvasive molecular imaging for a variety of applications, including the detection and quantification of various bioluminescent or fluorescent reporters in cells or tissues (in phantoms, cultures, or small animals). The Optical Imaging Core offers assistance with experimental design, regulatory approval, troubleshooting, data management, analysis, and display.

Instrumentation

• Revvity IVIS Spectrum (John Morgan Building, Room B100, SCTR 1-133, and SCTR 6-124 behind SCXC barrier)

• LI-COR Pearl Trilogy (John Morgan Building, Room B100 and SCTR 1-133)

• Revvity IVIS Lumina III (SCTR 6-124 behind SCXC barrier)

• Curadel FLARE® (SCTR 1-133)

• Photon etc IR VIVO (John Morgan Building, Room B100)

• TriFoil Imaging InSyTe FLECT/CT (SCTR 1-132)

Services

• Revvity IVIS Lumina III
  • Advanced in vitro and in vivo bioluminescence and fluorescence imaging system
  • Seven fluorescence excitation and 19 emission filters
  • Wide-field lens adapter allowing imaging of up to five mice simultaneously
• Revvity IVIS Spectrum
  • Advanced in vitro and in vivobioluminescence and fluorescence imaging system
  • 10 fluorescence excitation filters and 20 fluorescence emission filters
  • Spectral unmixing and 3D imaging capability
• LI-COR Pearl Trilogy
  • In vitro and in vivo fluorescence and bioluminescence imaging
  • Simultaneous detection of 700 nm and 800 nm channels
• Curadel FLARE
  • Open surgery to digital pathology
  • Simultaneous acquisition of color videos, 700 and 800 nm NIR fluorescence
  • High sensitivity, high resolution, high specificity imaging and quantification of two independent targets
• Photon etc IR VIVO
  • SWIR (NIR-II) imaging technology
  • Equipped with 4 laser sources (670, 760, 808 and 890 nm) and the emission spectral range is 850-1620 nm
  • An ultra-low noise InGaAs camera to provide optimized contrast resolution and sensitivity
  • High spatial and temporal resolution, and good penetration depth
• TriFoil InSyTe FLECT/CT
  • A dual modality imager that combines 3D fluorescence imaging with microCT imaging. 
  • Combined with the inline X-ray CT, the system provides 3D fluorescence tomographic imaging with anatomical reference
  • Equipped with 4 laser sources with a detection spectral range of 320-1100 nm

Assistance provided per user request: 

• General advice on experimental design or protocol plans

• Rodent anesthesia

• Animal shaving

• Living Image and Image Studio acquisition

• Data quantification and analysis

• Transferring Living Image/Image Studio data to other file formats

Rates and Scheduling

The core is set up to provide service at fee with no profit built in. The fees charged include costs for current personnel, operation, necessary equipment maintenance and upgrades, etc.

Rates: As of July 1, 2021, usage rates are $135 per hour for the IVIS Spectrum and the IVIS Lumina III, $90 per hour for the LI-COR Pearl Trilogy, Photon etc IR VIVO, and the Curadel Flare. Data analysis and training is free of charge. Limited free time is available on the InSyTe FLECT-CT to establish new protocols. . 

Contact Information

Jim Delikatny, PhD
Director, Optical Bioluminescence Sub-Core and Research Professor of Radiology
Phone: 215-898-3105
Email: delikatn@pennmedicine.upenn.edu

Ching-Hui Huang, PhD 
Technical Director, Optical Bioluminescence Sub-Core
Phone: 215-746-1427
Email: chinghu@pennmedicine.upenn.edu