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John S. Riley, MD, MS

John Riley
Medical School: Perelman School of Medicine at the University of Pennsylvania Undergraduate: Harvard University

About Dr. Riley

John Riley is a seventh-year general surgery resident at the Hospital of the University of Pennsylvania. After residency, he will begin fellowship training in pediatric surgery at the Children’s Hospital of Philadelphia. 

Education

2013 – 2018
MD - Perelman School of Medicine at the University of Pennsylvania
Philadelphia, Pennsylvania

2016 – 2018
MS - Translational Research – University of Pennsylvania
Philadelphia, Pennsylvania

2012 – 2013
MSt - Greek/Roman History – Oxford University
Oxford, UK

2006 – 2010
BA - Harvard University
Cambridge, Massachusetts

Societies/Memberships

  • Alpha Omega Alpha
  • Gold Humanism Society
  • Phi Beta Kappa
  • American College of Surgeons, Resident Member

Awards

2023
2nd Place Award, Five-minute Thesis Competition
American Society of Gene and Cell Therapy

2nd Place Award, Annual Surgical Trainee Paper Competition
Philadelphia Academy of Surgery

2022
Top Gun Award
Department of Surgery, Hospital of the University of Pennsylvania

Penn Center for Surgical Excellence Award
Department of Surgery, Hospital of the University of Pennsylvania

2021
Resident Research Scholarship
American College of Surgeons

Ruth L. Kirschstein National Research Service Award Individual Postdoctoral Fellowship
Eunice Kennedy Shriver National Institute of Child Health & Human Development of the National Institutes of Health

2019
M. Judah Folkman Award
American Pediatric Surgical Association Annual Meeting

2018
Clyde F. Barker Research Prize
Perelman School of Medicine at the University of Pennsylvania

2017
President’s Prize
British Association of Paediatric Surgeons Annual International Congress

Pediatric Research Prize
Perelman School of Medicine at the University of Pennsylvania

2013
Twenty-first Century Scholarship
Perelman School of Medicine at the University of Pennsylvania

2010
Francis H. Burr Scholarship
Harvard University

2009
Newbold Rhinelander Landon Memorial Scholarship
Harvard University

Research

07/2021 - 06/2023
Center for Fetal Research
The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania
Postdoctoral Research Fellow, William H. Peranteau, MD

02/2014 - 05/2018
Center for Fetal Research
The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania
Student Researcher, William H. Peranteau, MD

06/2015 - 05/2018
Pulmonary Hypoplasia Program
The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania
Student Researcher, Holly L. Hedrick, MD

01/2012 - 08/2012
Division of Endocrinology
Beth Israel Deaconess Medical Center, Boston, Massachusetts
Research Assistant, Mark A. Herman, MD

Publications

Peer-Reviewed

  1. Riley JS, Luks VL, Berkowitz CL, Dumitru AM, Kus NJ, Dave A, Menon P, De Paepe ME, Jain R, Li L, Dugoff L, Paidas-Teefey C, Alameh MG, Zoltick PW, Peranteau WH. Pre-existing maternal immunity to AAV but not Cas9 endonuclease impairs in utero gene editing in mice. Journal of Clinical Investigation. 2024;134(12):e179848.
  2. Riley JS, Berkowitz CL, Luks VL, Dave A, Cyril-Olutayo MC, Pogoriler J, Flake AW, Abdulmalik O, Peranteau WH. Immune modulation permits tolerance and engraftment in a mouse model of late-gestation transplantation. Blood Advances. 2024;8(17):4523-4538.
  3. Palanki R*, Riley JS*, Bose SK, Luks VL, Dave A, Kus NJ, White BM, Ricciardi AS, Swingle KL, Xue L, Sung DC, Thatte A, Safford HC, Chaluvadi VS, Carpenter M, Han EL, Maganti R, Hamilton AG, Mrksich K, Billingsley MM, Zoltick P, Alameh MG, Weissman D, Mitchell MJ, Peranteau WH. In utero delivery of targeted ionizable lipid nanoparticles facilitates in vivo gene editing of hematopoietic stem cells. Proceedings of the National Academy of Sciences. 2024;121(32):e2400783121.
  4. Riley JS, Schomberg J, Mantha A, Guner YS, Cuevas A, Stephenson CD, Peranteau WH, Yu PT. A novel VACTERL assessment tool to facilitate counseling for expectant families. Fetal Diagnosis and Therapy. 2023;50(5):368-375.
  5. Riley JS, McClain LE, Stratigis JD, Coons BE, Bose SK, Dave A, White BM, Li H, Loukogeorgakis SP, Fachin CG, Dias, AIBS, Flake AW, Peranteau WH. Fetal allotransplant recipients are resistant to graft-versus-host disease. Experimental Hematology. 2023;11:31-39.e3.
  6. Riley JS, Luks VL, Pina LF, Al Adas Z, Stoecker JB, Jackson BM, Braslow BM, Holena DN. COVID-19 pandemic significantly decreases acute surgical complaints. American Surgeon. 2020;86(11):1492-1500.
  7. Riley JS, McClain LE, Stratigis JD, Coons BE, Ahn NJ, Li H, Loukogeorgakis SP, Fachin CG, Dias AIBS, Flake AW, Peranteau WH. Regulatory T cells promote alloengraftment in a model of late-gestation in utero hematopoietic cell transplantation. Blood Advances. 2020;4(6):1102-1114.
  8. Riley JS, Antiel RM, Flake AW, Johnson MP, Rintoul NE, Lantos JD, Traynor MD, Adzick, NS, Feudtner C, Heuer GG. Pediatric neurosurgeons' views regarding prenatal surgery for myelomeningocele and the management of hydrocephalus: a national survey. Neurosurgical Focus. 2019;47(4):E8.
  9. Riley JS, McClain LE, Stratigis JD, Coons BE, Li H, Hartman HA, Peranteau WH. Pre-Existing maternal antibodies cause rapid prenatal rejection of allotransplants in the mouse model of in utero hematopoietic cell transplantation. Journal of Immunology. 2018;201(5):1549-1557. doi: 10.4049/jimmunol.1800183.
  10. Riley JS, Urwin JW, Oliver ER, Coleman BG, Khalek N, Moldenhauer JS, Spinner SS, Hedrick HL, Adzick NS, Peranteau WH. Prenatal growth characteristics and pre/postnatal management of bronchopulmonary sequestrations. Journal of Pediatric Surgery. 2018;53:265-269.
  11. Riley JS, Antiel RM, Rintoul NE, Ades AM, Waqar LN, Lin N, Herkert LM, D’Agostino JA, Hoffman C, Peranteau WH, Flake AW, Adzick NS, Hedrick HL. Reduced oxygen concentration for the resuscitation of infants with congenital diaphragmatic hernia. Journal of Perinatology. 2018;38:834-843.
  12. Riley JS, Bartlett EK, Dempsey DT. A left thoracoabdominal approach for transdiaphragmatic fistulas following bariatric surgery. Surgery for Obesity and Related Diseases. 2017;13(8):1455-1458.
  13. Baeza J, Coons BE, Lin Z, Riley JS, Mendoza M, Peranteau WH, Garcia BA. In utero pulse injection of isotopic amino acids quantifies protein turnover rates during murine fetal development. Cell Rep Methods. 2024;4(2):100713.
  14. Yu PT, Goodman LF, Riley JS, Giron A, Guner YS, Peranteau WH, Schomberg J. Racial disparity among youth decedents of legal intervention trauma. Journal of the American College of Surgeons. 2024;238(2):226-235.
  15. Dave A, Liu S, Riley JS, Bose S, Luks VL, Berkowitz CL, Menon P, Jung S, Kurre P, Peranteau WH. In utero hematopoietic cell transplantation leads to sustained engraftment in a mouse model of Fanconi Anemia. Blood Advances. 2023; 2024;8(3):624-628.\
  16. Dave A, Berkowitz CL, Luks VL, White BM, Palanki R, Carpenter MD, Riley JS, Bose SK, Li H, Li L, Menon PV, Teerdhala S, Ebrahimi M, Zoltick PW, Peranteau WH. Comment on: Premature delivery in the domestic sow in response to in utero delivery of AAV9 to fetal piglets. Gene Therapy. 2023;30(3-4):232-235.
  17. Burgos CM, Davey MG, Riley JS, Huimin J, Flake AW, Peranteau WH. Lung function and pulmonary artery blood flow following prenatal maternal retinoic acid and imatinib in the nitrofen model of congenital diaphragmatic hernia. Journal of Pediatric Surgery. 2017;53(9):1681-1687.
  18. Davey MG, Riley JS, Andrews A, Tyminski A, Limberis M, Pogoriler JE, Partridge E, Olive A, Hedrick HL, Flake AW, Peranteau WH. Induction of immune tolerance to foreign protein via adeno-associated viral vector gene transfer in mid-gestation fetal sheep. PLoS ONE. 2017;12(1): e0171132.
  19. Kim AG, Vrecenak JD, Boelig MM, Eissenberg L, Rettig MP, Riley JS, Holt MS, Conner MA, Loukogeorgakis SP, Li H, DiPersio JF, Flake AW, Peranteau WH. Enhanced in utero allogeneic engraftment in mice after mobilizing fetal HSCs by α4β1/7 inhibition. Blood. 2016;128:2457-2461.
  20. Burgos CM, Pearson EG, Davey M, Riley J, Jia H, Laje P, Flake AW, Peranteau WH. Improved pulmonary function in the nitrofen model of congenital diaphragmatic hernia following prenatal maternal dexamethasone and/or sildenafil. Pediatric Research. 2016;80:577-585.
  21. Antiel RM, Riley JS, Cahill PJ, Campbell RM, Waqar LN, Herkert LM, Rintoul NE, Peranteau WH, Flake AW, Adzick NS, Hedrick HL. Management and outcomes of scoliosis in children with congenital diaphragmatic hernia (CDH). Journal of Pediatric Surgery. 2016;51:1921-1925.

II. Abstracts – Oral

  1. Riley JS. Fetal gene editing and maternal immunity. Owen H. Wangensteen Scientific Forum, American College of Surgeons Clinical Congress. Boston, MA. October 24, 2023.
  2. Riley JS, Luks VL, Berkowitz CL, Dumitru AM, Dugoff L, Paidas-Teefey C, Zoltick PW, Peranteau WH. Characterizing pre-existing maternal immunity as a potential barrier to CRISPR-Cas9 in utero gene editing for metabolic liver diseases. International Fetal Medicine and Surgery Society Annual Meeting. Visby, Sweden. September 5, 2023.
  3. Riley JS, Luks VL, Berkowitz CL, Dave A, Zoltick PW, Peranteau WH. Pre-existing maternal immunity to adeno-associated virus causes fetal mortality after in utero gene editing mediated by maternal interleukin-6 and CXCL1. American Society of Gene and Cell Therapy Annual Meeting. Los Angeles, CA. May 18, 2023.
  4. Riley JS, Luks VL, Berkowitz CL, Dave A, Zoltick PW, Peranteau WH. Fetal gene editing evades maternal T cell immunity to Cas9 endonuclease. American Pediatric Surgical Association Annual Meeting. Orlando, FL. May 13, 2023.
  5. Riley JS, Dave A, Menon P, Luks VL, Berkowitz CL, Zoltick PW, Peranteau WH. Pre-existing maternal immunity to AAV but not Cas9 impairs in utero gene editing. International Fetal Medicine and Surgery Society Annual Meeting. Los Cabos, Mexico. November 10, 2022.
  6. Riley JS, Dave A, Berkowitz CL, Peranteau WH. Transient T cell depletion restores allograft receptivity in the mouse model of late-gestation in utero hematopoietic cell transplantation. International Fetal Medicine and Surgery Society Annual Meeting. Los Cabos, Mexico. November 10, 2022.
  7. Riley JS, Schomberg J, Mantha A, Guner YS, Cuevas A, Stephenson CD, Peranteau WH, Yu PT. A novel VACTERL association calculator to facilitate counseling for expectant families. International Fetal Medicine and Surgery Society Annual Meeting. Los Cabos, Mexico. November 8, 2022.
  8. Riley JS, Soriano IS. Robotic approach for the conversion of vertical banded gastroplasty to Roux-en-Y gastric bypass [video]. International Federation Surgery of Obesity World Congress. Miami, FL. August 26, 2022.
  9. Riley JS, Dave A, Luks VL, Zoltick PW, Peranteau WH. Pre-existing maternal humoral immunity to adeno-associated virus impairs fetal gene editing in a serotype-specific fashion. American Society of Gene and Cell Therapy Annual Meeting. Washington, DC. May 17, 2022.
  10. Riley JS, Dave A, Berkowitz CL, Peranteau WH. ‘Turning back the clock’: transient T cell depletion restores allograft receptivity in the mouse model of late-gestation in utero hematopoietic cell transplantation. American Pediatric Surgical Association Annual Meeting. San Diego, CA. May 13, 2022.
  11. Riley JS, Stratigis JD, McClain LE, Ahn NJ, Coons BE, Li H, Flake AW, Peranteau WH. Extending the ‘window of tolerance’: regulatory T cells permit late-gestation in utero hematopoietic cell transplantation. American Pediatric Surgical Association Annual Meeting, Boston, MA. May 22, 2019.
  12. Riley JS, Urwin JW, Oliver ER, Coleman BG, Johnson MP, Khalek N, Moldenhauer J, Howell LJ, Spinner SS, Hedrick HL, Flake AW, Adzick NS, Peranteau WH. Prenatal growth characteristics and pre/postnatal management of bronchopulmonary sequestrations. International Fetal Medicine and Surgery Society Annual Meeting. Jackson Hole, WY. October 8, 2017.
  13. Riley JS, McClain LE, Stratigis JD, Li H, Flake AW, Peranteau WH. Regulatory T cells prevent donor cell rejection in a mouse model of delayed gestational age in utero hematopoietic cell transplantation. International Fetal Medicine and Surgery Society Annual Meeting. Jackson Hole, WY. October 8, 2017.
  14. Riley JS, Urwin JW, Oliver ER, Coleman BG, Johnson MP, Khalek N, Moldenhauer JS, Gebb J, Howell LJ, Hedrick HL, Flake AW, Adzick NS, Peranteau WH. Prenatal growth characteristics and pre/postnatal management of prenatally-diagnosed extralobar bronchopulmonary sequestrations. British Association of Paediatric Surgeons Annual International Congress. London, UK. July 19, 2017.
  15. Riley JS, McClain LE, Li H, Stratigis JD, Flake AW, Peranteau WH. Maternal IgG can cause prenatal graft rejection in the mouse model of in utero hematopoietic cell transplantation. Meeting of the International Fetal Immunology and Transplantation Society. Philadelphia, PA. March 23, 2017.
  16. Giron A, Goodman L, Riley J, Guner Y, Peranteau W, Schomberg J, Yu P. Racial Disparity Among Youth Decedents of Legal Intervention Trauma. 4th Annual UCI Department of Surgery Research Symposium. Irvine, CA. Aug 22, 2024.
  17. Dumitru AM, Berkowitz CL, Whittake M, Wang X, Kus N, Luks VL, Riley JS, Carpenter M, Archer NF, Brooks D, Johnston W, Alameh MG, Musunuru K, Peranteau WH. Optimizing therapeutic base editing for hereditary tyrosinemia type 1. American Society of Gene and Cell Therapy Annual Meeting. Los Angeles, CA. May 18, 2023.
  18. Berkowitz CL, Dave A, Luks VL, Menon PV, Carpenter MD, Riley JS, Alameh MG, Peranteau WH. Intracranial Delivery of Lipid Nanoparticles in the Murine Model. American College of Surgeons Clinical Congress. San Diego, CA. October 18, 2022.
  19. Goodman LF, Schomberg J, Wallace EL, Guner YS, Riley JS, Peranteau WH, Yu PT. African American youths are more frequently subject to lethal force during legal intervention. American Pediatric Surgical Association Annual Meeting. San Diego, CA. May 12, 2022.
  20. Grant MT, MacGregor RM, Riley JS, Blinman T, Yu PT, Shakhsheer B, Antiel RM. Incidence and length of stay for pediatric appendicitis during the initial COVID-19 pandemic. Academic Surgical Congress. Orlando, FL. February 1, 2022.
  21. Antiel RA, Riley JS, Traynor Jr. MD, Flake AW, Johnson MP, Rintoul NE, Lantos JD, Heuer G, Feudtner C. Neurosurgeon views regarding prenatal surgery for myelomeningocele. Pacific Association of Pediatric Surgeons Annual Scientific Meeting. Christchurch, New Zealand. March 11, 2019.
  22. McGovern PE, Stratigis JD, Tuttle J, Riley JS, Ahn NJ, Flake AW, Peranteau WH. C-kit directed immunotoxins improve allogeneic engraftment after fetal hematopoietic stem cell transplantation. American Pediatric Surgical Association. Palm Desert, CA. May 3, 2018.
  23. McGovern P, Czechowicz A, Stratigis J, Li H. Zoltick P, Riley JS, Flake AW, Peranteau WH. Leukocyte-specific conditioning after tolerance induction via intra-uterine hematopoietic stem cell transplantation. Meeting of the International Fetal Immunology and Transplantation Society. Philadelphia, PA. March 24, 2017.
  24. Antiel RM, Riley JS, Cahill PJ, Campbell RM, Wagar LN, Herkert LM, Rintoul NE, Peranteau WH, Flake AW, Adzick NS, Hedrick HL. Management and outcomes of scoliosis in children with congenital diaphragmatic hernia (CDH). Pacific Association of Pediatric Surgeons. Koloa, HI. April 27, 2016.
  25. Antiel RM, Riley JS, Rintoul NE, Ades AM, Waqar LN, Herkert LM, Peranteau WH, Flake AW, Adzick NS, Hedrick HL. Oxygen concentration during resuscitation and requirement for ECMO and survival in congenital diaphragmatic hernia (CDH). 32nd Annual Children's National Symposium: ECMO and the Advanced Therapies for Respiratory Failure. Keystone, CO. March 2, 2016.

III. Abstracts – Poster

  1. Riley JS, Luks VL, Berkowitz CL, Dumitru AM, Dugoff L, Paidas-Teefey C, Zoltick PW, Peranteau WH. Characterizing Pre-existing Maternal Immunity as a Potential Barrier to CRISPR-Cas9 In Utero Gene Editing for Metabolic Liver Diseases. Ali Naji Research Day. Philadelphia, PA. June 15, 2023.
  2. Riley JS, Luks VL, Berkowitz CL, Dumitru AM, Zoltick PW, Peranteau WH. CRISPR-Cas9 in utero correction of murine tyrosinemia is not impaired by pre-existing maternal immunity to Cas9 endonuclease. American Society of Gene and Cell Therapy Annual Meeting. Los Angeles, CA. May 17, 2023.
  3. Riley JS, Dave A, Abdulmalik OY, Peranteau WH. Late-gestation hematopoietic cell transplantation with transient T cell depletion significantly improves disease phenotype in the Townes mouse model of Sickle Cell disease. American Society of Hematology. New Orleans, LA. December 12, 2022.
  4. Riley JS, Dave A, Menon P, Luks VL, Berkowitz CL, Zoltick PW, Peranteau WH. Maternal Cas9 antibodies do not prevent fetal gene editing. American College of Surgeons Clinical Congress. San Diego, CA. October 18, 2022.
  5. Riley JS, Antiel RM, Flake AW, Johnson MP, Rintoul NE, Lantos J, Feudtner C. Neurosurgeon views regarding prenatal surgery for myelomeningocele. Pediatric Academic Societies. Toronto, ON. May 5, 2018.
  6. Riley JS, McClain LE, Stratigis J, Li H, Flake AW, Peranteau WH. Regulatory T cells prevent donor cell rejection in a mouse model of delayed gestational age in utero hematopoietic cell transplantation. American Society of Hematology. Atlanta, GA. December 11, 2017. 
  7. Riley JS, McClain LE, Lee G, Li H, Flake AW, Peranteau WH. Characterizing and augmenting peripheral tolerance in in utero hematopoietic cell transplantation. American Society of Hematology. San Diego, CA. December 5, 2016.
  8. Riley JS, Antiel RM, Rintoul NE, Ades AM, Waqar LN, Herkert LM, Peranteau WH, Flake AW, Adzick NS, Hedrick HL. Reduced oxygen concentration for the resuscitation of infants with congenital diaphragmatic hernia. Pediatric Academic Societies. Baltimore, MD. May 3, 2016.
  9. Johnston W, Carpenter M, Riley JS, Luks VL, Berkowitz CL, Kus N, Dumitru AM, Palanki R, Alameh MG, Peranteau WH. In utero lipid nanoparticle targeting of the fetal skin for mRNA therapies. American Society of Gene and Cell Therapy Annual Meeting. Los Angeles, CA. May 17, 2023.
  10. Luks VL, Dave A, Berkowitz CL, Riley JS, Carpenter M, Zoltick PW, Peranteau WH. In utero pulmonary gene editing persists after postnatal lung injury. American Society of Gene and Cell Therapy Annual Meeting. Los Angeles, CA. May 17, 2023.
  11. Dave A, Liu S, Riley JS, Kurre P, Peranteau WH. Sustained engraftment in Fanconi Anemia mice using in utero hematopoietic cell transplantation. American Society of Hematology. New Orleans, LA. December 11, 2022.
  12. Luks VL, Dave A, Berkowitz CL, Puc M, Riley JS, Carpenter MD, Alameh MG, Peranteau WH. Targeting Of Fetal And Neonatal Pulmonary Epithelial Cells For Therapeutic Applications. American College of Surgeons Clinical Congress. San Diego, CA. October 18, 2022.
  13. Dave A, Liu S, Bose SK, Riley JS, Menon P, Berkowitz CL, Luks VL, Li H, Kurre P, Peranteau WH. Fanconi Anemia mice maintain long-term engraftment after in utero hematopoietic cell transplantation. Fanconi Anemia Research Fund Scientific Symposium. Austin, TX. September 8, 2022.
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